Validation of high-affinity binding sites for succinic acid through distinguishable binding of γ-hydroxybutyric acid receptor-specific NCS 382 antipodes

γ-hydroxybutyric acid (GHB) binding to multiple sites for the tricarboxylic acid cycle intermediate succinic acid(SUC) has been disclosed recently. In order to better characterize these targets, distinguishable binding of GHB receptor-specific NCS 382 antipodes to [(3)H]-SUC or [(3)H]-GHB labelled sites in rat brain synaptic membranes was explored. Eutomer binding parameters suggest identity of the high-affinity target for SUC with a synaptic GHB receptor subtype.


Tünde Molnár, Júlia Visy, Ágnes Simon, István Moldvai, Eszter Temesvári-Major, Gábor Dörnyei, Erzsébet Kútiné Fekete, Julianna Kardos
December 1, 2008
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